Toxin Repertoire of the Deep-Sea Zoantharian Zibrowius primnoidus (Cnidaria: Zoantharia)

This BioProject presents the transcriptomic analysis of the deep-sea zoantharian Zibrowius primnoidus, collected at 255 m depth in the Sicily Channel, Italy, to characterize its toxin repertoire using a bulk RNA-seq approach. Twelve polyps were preserved in RNA later, washed in PBS, and processed using a TRIzol-based RNA extraction with magnetic beads. After purification (Mag-Bind Total Pure NGS kit), RNA integrity was assessed via TapeStation (High Sensitivity RNA Screen Tape Assay). Library preparation used the Zymo-Seq RiboFree Total RNA Kit for rRNA depletion, followed by paired-end 150 bp sequencing on an Illumina NovaSeq 6000, generating 90,741,564 raw reads. Primary bioinformatics included base calling, demultiplexing, and adapter masking with Illumina BCL Convert v3.9.31. This dataset provides insights into the molecular adaptations and venom composition of Zibrowius primnoidus, contributing to the study of deep-sea cnidarian toxins.

本生物项目（BioProject）针对在意大利西西里海峡255米水深采集的深海群海葵（zoantharian）*Zibrowius primnoidus*开展转录组分析，旨在通过批量RNA测序（bulk RNA-seq）技术解析其毒素组特征。研究人员将12个水螅体（polyp）置于RNA保存液（RNA later）中保存，经磷酸盐缓冲液（PBS）清洗后，采用基于TRIzol（TRIzol）试剂结合磁珠的方法完成RNA提取。经Mag-Bind Total Pure NGS试剂盒（Mag-Bind Total Pure NGS kit）纯化后，通过TapeStation（TapeStation）系统搭配高灵敏度RNA检测试剂盒（High Sensitivity RNA Screen Tape Assay）对RNA完整性进行评估。文库构建采用Zymo-Seq RiboFree总RNA试剂盒（Zymo-Seq RiboFree Total RNA Kit）完成核糖体RNA（rRNA）去除，随后在Illumina NovaSeq 6000（Illumina NovaSeq 6000）测序平台上开展双端150bp测序，共产出90,741,564条原始读段（raw reads）。初步生物信息学分析环节采用Illumina BCL Convert v3.9.31（Illumina BCL Convert v3.9.31）软件完成碱基识别、样本解复用及接头序列屏蔽。本数据集可为*Zibrowius primnoidus*的分子适应性及毒液组分研究提供参考，助力深海刺胞动物（cnidarian）毒素相关研究。