Additional file 1 of Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens

Additional file 1. Results of the suspension array with sera from mice infected with various Chlamydia species. Pooled sera from mice experimentally infected with Chlamydia, and two pools from naive mice, were tested with bead sets loaded with antigens as indicated. Results for C. psittaci, C. gallinacea, and the last naive mouse are also presented in Figure 1. The results are given in MFI, values above the cutoffs are highlighted in red. For beads with the negative control (NC) compound, values given are the average MFI of three parallel runs; the standard deviations are also given (SD). Serum-specific cutoffs were calculated as three times the average signal on NC beads from three parallel runs (3*avNC). Abbreviations: p, purified peptide; Cga, Cps, Cab, and Cav resp. C. gallinacea, C. psittaci, C. abortus, C. avium. NB in the manuscript normalized data are used for which the cutoffs were subtracted from the acquired MFI values.

附加文件1：不同衣原体感染小鼠血清的悬浮点阵实验结果。本实验对经衣原体人工感染小鼠的混合血清，以及两份未感染健康小鼠的混合血清，采用负载有指定抗原的微球试剂盒进行检测。鹦鹉热衣原体（C. psittaci）、鸡衣原体（C. gallinacea）以及最后一只未感染小鼠的检测结果亦见图1。检测结果以平均荧光强度（Mean Fluorescence Intensity, MFI）表示，高于临界值（cutoffs）的结果以红色标注。对于负载阴性对照（NC）复合物的微球，所给出的数值为三次平行实验的平均MFI，同时也标注了标准差（Standard Deviation, SD）。血清特异性临界值按照三次平行实验中NC微球的平均信号值的三倍计算（即3×avNC）。缩写说明：p代表纯化肽；Cga、Cps、Cab及Cav分别代表鸡衣原体（C. gallinacea）、鹦鹉热衣原体（C. psittaci）、流产衣原体（C. abortus）及禽衣原体（C. avium）。注：本文手稿中使用的是标准化后的数据，即从测得的MFI值中扣除临界值后得到的结果。