Inhibitory effect of Lonchocarpus araripensis lectin in rat acute models of inflammation

Abstract: Dalbergieae tribe lectins, possessing binding affinity for galactose and mannose, present inflammatory and nociceptive effects, while those for N-acetylglucosamine are anti-inflammatory. Since the anti-inflammatory effect of the seed lectin of L. araripensis (LAL) had been already demonstrated in mice, this effect was presently evaluated in rat models of acute inflammation. LAL (0.01-1 mg/kg) was administered by intravenous (i.v.) route in male Wistar rats 30 min before paw edema induction by dextran or carrageenan, and peritonitis by carrageenan. LAL (1 mg/kg) was incubated with N-acetylglucosamine for allowing lectin-sugar interactions before injection into animals. LAL toxicity was evaluated by the parameters: body mass, organs weight, stomach macroscopy, hematological and biochemical dosage. Statistical analysis was performed by ANOVA and Bonferroni’s test (p

摘要：对半乳糖和甘露糖具有结合亲和性的黄檀族（Dalbergieae tribe）凝集素，可表现出促炎与伤害感受效应；而靶向N-乙酰葡糖胺（N-acetylglucosamine）的凝集素则具备抗炎活性。此前已有研究证实阿鲁比花生（L. araripensis）种子凝集素（LAL）的抗炎作用在小鼠模型中得到验证，本研究旨在急性炎症大鼠模型中对该效应进行评估。实验中，雄性Wistar大鼠在经葡聚糖或角叉菜胶诱导足肿胀、经角叉菜胶诱导腹膜炎前30分钟，通过静脉（i.v.）途径给予0.01~1 mg/kg剂量的LAL。另有实验组将1 mg/kg的LAL与N-乙酰葡糖胺孵育，使其发生凝集素-糖相互作用后再注入动物体内。本研究通过体质量、脏器重量、胃大体观察、血液学与生化指标检测等参数评估LAL的毒性。统计学分析采用方差分析（ANOVA）与Bonferroni检验，以p值作为显著性判断依据。