BRE silencing effect on gene expression profile of human umbilical cord perivascular (HUCPV) progenitor cells. Homo sapiens

Analysis of HUCPV progenitor cells following BRE silencing using BRE-siRNA. BRE (Brain and reproductive organ-expressed) protein is also known as TNFRSF1A (tumor necrosis factor receptor superfamily, member 1A) modulator and BRCC45 (BRCA-1 containing complex subunit 45). Results identify gene targets of BRE. Overall design: Total RNA extracted from 3 separate cell cultures that have been transfected with BRE-siRNA and 3 separate cell cultures that have been transfected with ctl (scrambled)-siRNA.

采用BRE-siRNA沉默BRE基因后人脐带血祖细胞（HUCPV progenitor cells）的分析。BRE（脑与生殖器官表达蛋白）又名肿瘤坏死因子受体超家族成员1A（TNFRSF1A）调节蛋白以及含BRCA1复合物亚基45（BRCC45）。本研究鉴定出了BRE的靶基因。实验设计：分别从3份转染BRE-siRNA的独立细胞培养物，以及3份转染对照（乱序）-siRNA的独立细胞培养物中提取总RNA。