Rutidosis leptorrhynchoides Genome sequencing

The Rutidosis leptorrhynchoides (also spelled R. leptorhynchoides, Button Wrinklewort) genome project aims to produce a high-quality, telomere-to-telomere assembly from one diploid individual plants of Truganina Cemetery ancestry to support conservation efforts for this endangered south-eastern Australian species, threatened by habitat loss and fragmentation and genetic erosion. Plant 2 (leaves) was used for HiC, and long read sequencing on the PacBio Revio and ONT PromethION platforms. A second plant, Plant 1, was used for Illumina RNASeq (leaf, & stem). This project provides essential resources to improve our understanding of the species' genomic architecture, including self-incompatibility mechanisms crucial for reproduction. These resources will contribute to a broader study developing genomic data to inform genetic management strategies, including assisted gene flow between isolated populations. By enhancing genetic diversity and adaptive potential, this research aims to ensure the long-term survival and resilience of R. leptorrhynchoides in the face of environmental change.

细喙阔苞菊（Rutidosis leptorrhynchoides，亦拼写为R. leptorhynchoides，俗称纽扣皱皮菊（Button Wrinklewort））基因组项目，旨在从一株携带特鲁吉纳公墓（Truganina Cemetery）血统的二倍体植株中，构建高质量的端粒到端粒（telomere-to-telomere）基因组组装，以支撑这一受栖息地丧失、碎片化及遗传侵蚀威胁的澳大利亚东南部濒危物种的保护工作。本研究选取植株2的叶片组织开展高通量染色体构象捕获（HiC）实验，并基于PacBio Revio与ONT PromethION测序平台完成长读长测序；另一株植株（植株1）的叶片与茎组织则被用于Illumina RNA测序（RNASeq）。本项目提供的关键基因组资源，将助力我们深入解析该物种的基因组结构，包括对其繁殖至关重要的自交不亲和机制。这些资源将为一项更广泛的研究提供支撑，该研究将生成基因组数据以指导遗传管理策略制定，包括在隔离种群间开展辅助基因流工作。本研究旨在通过提升该物种的遗传多样性与适应潜力，保障细喙阔苞菊（R. leptorrhynchoides）在环境变化背景下的长期存续与恢复能力。