Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

This study investigated whether there are marked differences in surface markers between rabbit and human mesenchymal stem cells (MSCs). Murine and rabbit MSCs have been reported to be CD90-negative. Rat MSCs have been reported to be CD71-negative. Our previous study also shows that rabbit MSCs are CD29-negative. However, human MSCs are generally considered to be CD29-, CD71-, and CD90-positive. Therefore, the surface markers of human MSCs might differ from those of other species. Rabbit bone marrow MSCs were obtained that had a multi-differentiation potential. The phenotype of these cells was studied using flow cytometry antibodies for 25 rabbit surface markers, namely, CD13, CD14, CD29, CD31, CD34, CD44, CD45, CD49d, CD49f, CD51, CD54, CD59, CD71, CD73, CD90, CD105, CD106, CD133, CD166, MHC I, MHC II, α-smooth muscle actin (α-SMA), cytokeratin, desmin, and vimentin. The phenotype of commercially available human MSCs was similarly studied using antibodies for human surface markers. CD14, CD31, CD34, CD45, CD49d, CD49f, CD51, CD54, CD71, CD106, CD133, MHC II, and cytokeratin were absent from both rabbit and human MSCs, while CD44, α-SMA, and vimentin were present on both cell lines. CD13, CD29, CD59, CD73, CD90, CD105, CD166, and MHC I were present on human MSCs, but not on rabbit MSCs. However, desmin was present on rabbit MSCs, but not on human MSCs. In total, the surface expression of nine markers differed between human and rabbit MSCs, whereas the surface expression of 16 markers was the same in the two cell lines.

本研究旨在探究兔源与人源间充质干细胞（mesenchymal stem cells, MSCs）的表面标志物是否存在显著差异。已有研究报道，鼠源与兔源MSCs呈CD90阴性；大鼠源MSCs则被报道为CD71阴性。本团队前期研究亦证实，兔源MSCs为CD29阴性。然而，人源MSCs通常被认为是CD29阴性、CD71阴性且CD90阳性。据此推测，人源MSCs的表面标志物谱或与其他物种存在差异。本研究分离获取了具备多向分化潜能的兔骨髓MSCs，采用流式细胞术抗体，针对25种兔源表面标志物开展表型鉴定，所涉标志物包括CD13、CD14、CD29、CD31、CD34、CD44、CD45、CD49d、CD49f、CD51、CD54、CD59、CD71、CD73、CD90、CD105、CD106、CD133、CD166、主要组织相容性复合体I（MHC I）、主要组织相容性复合体II（MHC II）、α-平滑肌肌动蛋白（α-smooth muscle actin, α-SMA）、细胞角蛋白、结蛋白及波形蛋白。针对市售人源MSCs的表型，同样采用对应人源表面标志物抗体进行检测。结果显示，CD14、CD31、CD34、CD45、CD49d、CD49f、CD51、CD54、CD71、CD106、CD133、MHC II及细胞角蛋白在兔源与人源MSCs中均无表达；而CD44、α-SMA及波形蛋白在两类细胞中均有表达。CD13、CD29、CD59、CD73、CD90、CD105、CD166及MHC I仅在人源MSCs中表达，兔源MSCs未检测到这些标志物。但结蛋白仅在兔源MSCs中表达，人源MSCs无该标志物的表面表达。综上，人源与兔源MSCs共有9种表面标志物的表达存在差异，另有16种表面标志物在两类细胞中的表达模式一致。