Rapid Receptor-Clustering Assay To Detect Uropathogenic and Diarrheal Escherichia coli Isolates Bearing Adhesins of the Dr Family

Infections caused by Escherichia coli isolates expressing adhesins of the Dr family are associated with diarrhea and urinary tract infections, and these E. coli strains recognize the complement regulatory protein decay-accelerating factor (DAF) as their receptor. Clustering of the DAF receptor at the sites of bacterial adherence to epithelial cells is proposed as an alternative to PCR assay for rapid detection of Dr-positive E. coli.

表达Dr家族黏附素的大肠埃希菌（Escherichia coli）分离株引发的感染与腹泻及尿路感染相关，此类大肠埃希菌菌株以补体调节蛋白衰变加速因子（decay-accelerating factor，DAF）作为其受体。有研究提出，在细菌黏附上皮细胞的位点处出现的DAF受体聚集现象，可作为聚合酶链式反应（PCR）检测法的替代方案，用于快速检测Dr阳性大肠埃希菌。