mRNA expression analysis of the hippocampus in a vervet monkey model of fetal alcohol spectrum disorder. mRNA expression analysis of the hippocampus in a vervet monkey model of fetal alcohol spectrum disorder

Alcohol preferring vervet dams were permitted to ingest alcohol 4 days/week during the second half of gestation (e90-e165) and paired with sucrose matched controls. Cases and controls were sacrificed with subsequent hippocampal extraction at 5 months and 2 years. RNA was extracted according to the manufacturer's protocol and hybridized to the Affymetrix Rhesus Genechip microarray. We sought to identify pathways and genes involved in the development of fetal alcohol spectrum disorder in a non-human primate in order to develop an understanding of how this disorder may develop in humans. Overall design: 2x2 ANOVA, main effect=alcohol, main effect=age, main effect=interaction. Six replicates/group

将偏好饮酒的妊娠雌性黑长尾猴（vervet）在妊娠中后期（胚胎第90天至第165天）期间每周给予4天饮酒处理，并设置蔗糖匹配对照组。分别于5月龄和2岁时对饮酒组与对照组动物实施安乐死，随后提取海马组织。按照试剂盒制造商提供的操作流程提取总RNA，并与Affymetrix恒河猴基因芯片微阵列进行杂交。本研究旨在鉴定非人灵长类动物中参与胎儿酒精谱系障碍（fetal alcohol spectrum disorder, FASD）发生发展的通路与基因，以阐明该疾病在人类中的潜在发病机制。实验整体设计：采用2×2方差分析（ANOVA），主效应分别为酒精处理、年龄，以及二者的交互作用；每组设置6个生物学重复。