Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes in fetal and 2 weeks old rat islets

Unlike adult β-cells, fetal and neonatal islets are functional immature and have blunted glucose responsiveness and decreased insulin secretion in response to stimuli. Pancreatic islets are a mixture of different cell types. Study of transcriptomes from intact islets would identify novel molecular mechanisms controlling islet functional development. At e19 and 2 weeks of age, pancreatic islets were isolated and total RNA were extracted for RNA-Seq study. Gene expression profiles were compared in the study.与成年β细胞不同，胎儿及新生期胰腺胰岛处于功能未成熟状态，其葡萄糖应答反应减弱，且受外界刺激时胰岛素分泌水平降低。胰腺胰岛是多种细胞类型的混合体。对完整胰岛的转录组（transcriptome）进行研究，可阐明调控胰岛功能发育的全新分子机制。本研究在胚胎第19天（e19）及出生后2周时分离胰腺胰岛，提取总RNA用于RNA-Seq研究，并对基因表达谱开展了对比分析。