Comparison of flocculation ability by ETH-2 and PAM for wine process wastewater.

Three hundred µl ETH-2 strain culture broth (OD600 = 1.8) and 0.1% PAM were added into 5 ml wine process wastewater (COD: 4299.0 mg/L; pH = 4.0; SS = 315.0 mg/L), respectively. The cylinder was gently shaken, settled at room temperature for 5 min, and measured the OD550.

分别将300 μL OD₆₀₀为1.8的ETH-2菌株培养液与0.1%的PAM(聚丙烯酰胺，Polyacrylamide)加入至5 mL葡萄酒加工废水中，该废水的化学需氧量(Chemical Oxygen Demand, COD)为4299.0 mg/L、酸碱度(pH)为4.0、悬浮物(Suspended Solids, SS)含量为315.0 mg/L。随后轻轻摇晃容器，于室温下静置沉降5分钟，测定其OD₅₅₀值。