Mammalian-type amino acids in the PB2 protein of ostrich isolates.

aWe intratracheally inoculated A/duck/Vietnam/5001/04 (H5N1) into a 3-week-old ostrich and isolated virus from trachea, lungs, and brain on day 3 post-infection (for more details, see [10]). Viral RNA was isolated, and the PB2 gene was amplified by RT-PCR and cloned. We then sequenced the indicated numbers of PB2 clones. Results for PB2-627 and PB2-701 were already shown in Shinya et al.[10]. We here expanded our analysis to include PB2-591 in this study.
bMammalian-type amino acids are shown in bold and italics.

a. 我们对1只3周龄鸵鸟经气管内接种A/duck/Vietnam/5001/04 (H5N1)毒株，并于感染后第3天从其气管、肺及脑组织中分离病毒（详细步骤参见文献[10]）。提取病毒RNA后，通过反转录聚合酶链式反应（Reverse Transcription-Polymerase Chain Reaction, RT-PCR）扩增PB2基因并进行克隆，随后对指定数量的PB2克隆进行测序。PB2-627与PB2-701位点的相关结果已在Shinya等的研究[10]中公布，本研究将分析范围拓展至PB2-591位点。
b. 哺乳动物型氨基酸以粗斜体格式展示。