Gene expression profile at single cell level of notochordal-like cells (NLC) derived from human induced pluripotent stem cells (hiPSC) differentiation

Notochordal cells are considered as a rare cell type ensuring signaling function required for proper development and homeostasis in various tissues. We used single cell RNA sequencing (scRNA-seq) to characterize gene signature of notochordal-like cells and understand heterogeneity of co-emerging cell types during in vitro differentiation. Overall design: hiPS-NLC differentiating cells were dissociated and labelled with cell-multiplexing oligos (CMO) according to hiPSC line origin and treatment and analyzed using scRNAseq.

脊索细胞（Notochordal cells）被认为是一类稀有细胞类型，其介导的信号功能是多种组织正常发育与稳态维持所必需的。本研究采用单细胞RNA测序（scRNA-seq）对脊索样细胞的基因特征进行解析，并探究体外分化过程中共同出现的细胞类型的异质性。实验设计概述：我们根据人诱导多能干细胞系的来源及处理方式，对hiPS-NLC分化细胞进行解离，并用细胞多重标记寡核苷酸（CMO）进行标记，随后通过scRNA-seq开展测序分析。