Transcriptomic Diversity of Innate Lymphoid Cells in Human Lymph nodes

Presence and functions of ILCs in the human draining lymph nodes (LN) are unknown. Here, we performed single-cell RNA sequencing to unravel the tissue-specific transcriptional profiles of ILCs compared them with that of bone marrow (BM) and spleen. Single cells from Lymph nodes, Bone marrow, and Spleen were captured using Multi Tissue Dissociation kit. Then the total innate lymphocyte were isolated using Flourecense activated cell sorting (FACS) according to the presense or acceptance of the CD7 marker.

人类引流淋巴结（Lymph Nodes, LN）中固有淋巴细胞（Innate Lymphoid Cells, ILCs）的存在情况与功能尚未明确。本研究采用单细胞RNA测序技术，解析固有淋巴细胞的组织特异性转录组特征，并将其与骨髓（Bone Marrow, BM）及脾脏中的对应转录组特征进行对比。研究人员使用多组织解离试剂盒（Multi Tissue Dissociation Kit）分离获取来自淋巴结、骨髓与脾脏的单细胞，随后依据CD7标志物的表达与否，通过荧光激活细胞分选术（Fluorescence Activated Cell Sorting, FACS）分离得到总固有淋巴细胞。