Methylation clocks for age estimation in toothed whales and dolphins. Methylation clocks for age estimation in toothed whales and dolphins

An Infinium microarray platform (GPL28271, HorvathMammalMethylChip40) was used to generate DNA methylation data from several tissues (skin, blood) in toothed whales and dolphins. Tissues: skin and blood. Overall design: N=545 tissue samples from 9 different toothed whale species were profiled on the mammalian methylation array platform (GPL28271). N= 238 skin tissue. The remaining samples come from blood (N=283), blubber (N=5), heart (2), kidney (2), liver (2), lung (2), lymphnode (2), muscle (2), pancreas (2), spleen (3), testis (1), thyroid (1). We profiled samples from the following 9 different toothed whale species whose Latin names are as follows: Cephalorhynchus commersonii (Commerson's dolphin), Delphinapterus leucas (Beluga), Delphinus delphis (Common dolphin), Globicephala macrorhynchus (Short-finned pilot whale), Lagenorhynchus obliquidens (Pacific white-sided dolphin), Orcinus orca (Killer whale), Phocoena phocoena (Harbor porpoise), Steno bredanensis (Rough-tooth dolphin), Tursiops truncatus (Bottlenose dolphin). Genome annotations of the array can be downloaded from https://github.com/shorvath/MammalianMethylationConsortium

本研究采用Infinium微阵列平台（GPL28271，HorvathMammalMethylChip40），对齿鲸与海豚的多种组织（皮肤、血液）开展DNA甲基化数据的获取工作。
组织类型：皮肤与血液。
实验整体设计：基于该哺乳动物甲基化芯片平台（GPL28271），共对来自9个不同齿鲸物种的545份组织样本进行了检测分析。
其中皮肤组织样本共238份。
剩余样本来源如下：血液283份、鲸脂5份、心脏2份、肾脏2份、肝脏2份、肺脏2份、淋巴结2份、肌肉2份、胰腺2份、脾脏3份、睾丸1份、甲状腺1份。
本次检测覆盖以下9个齿鲸物种，其拉丁学名与对应俗名如下：康氏矮海豚（Cephalorhynchus commersonii）、白鲸（Delphinapterus leucas）、普通真海豚（Delphinus delphis）、短肢领航鲸（Globicephala macrorhynchus）、太平洋白边海豚（Lagenorhynchus obliquidens）、虎鲸（Orcinus orca）、港湾鼠海豚（Phocoena phocoena）、糙齿海豚（Steno bredanensis）、宽吻海豚（Tursiops truncatus）。
该芯片的基因组注释信息可从以下网址下载：https://github.com/shorvath/MammalianMethylationConsortium