Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Tet2 Mutant Macrophage Transcriptomes [RAW264.7]. Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Tet2 Mutant Macrophage Transcriptomes [RAW264.7]

We used RNA-seq to detect the genes affected by Tet2 or Itaconate (ITA) treatment Overall design: WT and Tet2 knockout RAW264.7 were pretreated with octy-itaconate and induced by LPS and each treatment has three biological replicates

我们采用RNA测序（RNA-seq）技术，检测受Tet2或衣康酸（Itaconate, ITA）处理影响的基因。实验整体设计：将野生型（Wild Type, WT）与Tet2基因敲除的RAW264.7细胞先用辛基衣康酸进行预处理，随后经脂多糖（Lipopolysaccharide, LPS）诱导；每组处理设置3个生物学重复。