Next Generation Sequencing upon siRNA-mediated knockdown of EIF5A in MCF-7 cells.

The goal of this study is to examine the effect of EIF5A knockdown on the MCF-7 transcriptome. MCF-7 total RNA profiles upon siRNA-mediated knockdown of EIF5A or using a control siRNA (siCTRL) were generated by deep sequencing, in triplicate, using the Illumina TruSeq kit (RPHMR12126). Library products were sequenced on the Illumina NextSeq500 platform.

本研究旨在探究EIF5A基因敲降对MCF-7细胞转录组的影响。实验分别通过小干扰RNA（small interfering RNA, siRNA）介导EIF5A敲降，以及使用对照siRNA（siCTRL）处理MCF-7细胞；随后采用Illumina TruSeq建库试剂盒（货号RPHMR12126）对两组细胞的总RNA进行建库，并进行深度测序，设置三次生物学重复。所得文库产物在Illumina NextSeq500测序平台上完成测序。