Downregulation of MED12 via siRNA in SPHEROIDS derived from prostate cancer cell lines (3’ tag DGE). Downregulation of MED12 via siRNA in SPHEROIDS derived from prostate cancer cell lines (3’ tag DGE)

The Mediator complex is a multi-subunit protein that modulates gene expression on a genome-wide scale. MED12 and cyclin-dependent kinase 8 (CDK8) or its paralog CDK19 are components of its kinase module that regulate the proliferation of prostate cancer cells. In this study, we investigated how MED12 and CDK8/19 influence cancer-driven processes in prostate cancer cell lines, focusing on AR activity and enzalutamide resistance. Overall design: To investigate the relevance of MED12 in spheroids derived from enzalutamide sensitive and resistant prostate cancer cells, we performed siRNA mediated knockdown, generated spheroids and analyzed the response using high throughput 3' tag differential gene expression analysis.

中介体复合物（Mediator complex）是一种多亚基蛋白质，可在全基因组层面调控基因表达。MED12与细胞周期蛋白依赖性激酶8（cyclin-dependent kinase 8, CDK8）及其旁系同源物CDK19是其激酶模块的组成成分，该模块可调控前列腺癌细胞的增殖。本研究旨在探究MED12与CDK8/19如何影响前列腺癌细胞系中的癌症驱动进程，重点关注雄激素受体（Androgen Receptor, AR）活性与恩扎卢胺耐药性。实验整体设计：为探究MED12在恩扎卢胺敏感与耐药前列腺癌细胞来源的细胞球体中的相关作用，我们开展了siRNA介导的基因敲减实验，构建了细胞球体，并通过高通量3'标签差异基因表达分析手段检测其响应情况。