CIL:19124, Spiroplasma kunkelii, Zea mays, plant cell, prokaryotic cell. In Cell Image Library

Detailed methods: Tissue was processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. Abbreviations: n = nucleus, pa = parenchyma, ph = phloem, S = CSS, xy = xylem.

详细实验方法：将组织经戊二醛（glutaraldehyde）与四氧化锇（osmium tetroxide）固定后，采用Spurr包埋剂（Spurr's medium）进行包埋。超薄切片经醋酸铀（uranyl acetate）和柠檬酸铅（lead citrate）染色后，使用飞利浦-201型透射电子显微镜（Philips-201 TEM，非数码相机）进行观测。后续对拍摄所得的底片进行扫描，以生成本数据集的电子文件。缩写说明：n代表细胞核（nucleus），pa代表薄壁组织（parenchyma），ph代表韧皮部（phloem），S代表CSS，xy代表木质部（xylem）。