Vigor and oxidation reactions in soybean seedlings submitted to different seed chemical treatments

Abstract: The interaction of different seed chemical treatment with seedlings germination, vigor and stress metabolism attributes needs to be better understood in soybean crop. The objectives of this study were to evaluate seed vigor, germination, and biochemical attributes of soybean seedlings as a function of different seed chemical treatments. A completely randomized experimental design was adopted, with 8 seed treatments and 4 replicates. Seed germination, vigor (first germination count, cold test, accelerated aging, emergence speed index), seedling biomass, enzimes activity and SVIS® analysis were evaluated. The evaluated treatments had no difference relative to control on seed germination and vigor. Through image analysis, the seedling uniformity index were equal or higher than control with the seed chemical treatment, while vigor and growth indexes had no difference. Shoot biomass of seedlings tends to decrease with seed chemical treatments, and root biomass had no difference. Seed chemical treatments increased the activity of hydrogen peroxide in seedlings by 6 to 40% compared to control; the activity of catalase increased by 87 to 184% compared to control; and no effect was observed in lipids peroxidation. Overall, seed chemical treatment benefited the physiological activity of soybean seedlings and improved antioxidant metabolism activity. None of the seed chemical treatments used were phytotoxic.

摘要：大豆作物中，不同种子化学处理与幼苗萌发、活力及胁迫代谢相关性状间的互作机制仍有待进一步阐明。本研究旨在探究不同种子化学处理对大豆幼苗种子活力、萌发特性及生化性状的影响。本研究采用完全随机试验设计，设置8个种子处理组，每组4次重复。试验测定指标包括种子萌发率、活力指标（初始萌发计数、低温发芽试验、加速老化试验、出苗速率指数）、幼苗生物量、酶活性以及SVIS®分析。各供试种子处理组在种子萌发率与活力水平上与对照组无显著差异。通过图像分析可知，经种子化学处理的幼苗均匀度指数与对照组持平或更高，而活力与生长指数则无显著差异。幼苗地上部生物量随种子化学处理呈现下降趋势，而地下部根系生物量则无显著变化。与对照组相比，种子化学处理使幼苗体内过氧化氢活性提升6%~40%，过氧化氢酶活性提升87%~184%，但对脂质过氧化无显著影响。总体而言，种子化学处理可提升大豆幼苗的生理活性，强化抗氧化代谢能力，且所有供试种子化学处理均未表现出植物毒性。