An Ribonuclease T2 Family Protein Modulates Acinetobacter baumannii Abiotic Surface Colonization

A. baumannii has the propensity to colonize abiotic surfaces and this is thought to mediate its transmission to susceptible patients. We found that disruption of A. baumannii ribonuclease T2 family protein (ATCC 17978 locus A1S_3026) severely diminishes the organism's ability to colonize abiotic surfaces. We used Affymetrix A. baumannii GeneChips (part number PMDACBA1) to compare the gene expression properties of wild type and isogenic ribonuclease T2 family protein mutant cells. A. baumanni strain 98-37-09 (wild type) or isogenic ACJ7 (harboring a EZ-Tn5 insertion in A1S_3026) cells were grown to mid-exponential phase growth in Luria Burtani medium, total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the regulatory effects of A1S_3026.

鲍曼不动杆菌（Acinetobacter baumannii，下文简称A. baumannii）具有定殖非生物表面的特性，该特性被认为介导了其向易感患者的传播。我们发现，鲍曼不动杆菌核糖核酸酶T2家族蛋白（ribonuclease T2 family protein，ATCC 17978菌株基因座A1S_3026）的基因破坏，会显著削弱该菌定殖非生物表面的能力。本研究采用Affymetrix公司的鲍曼不动杆菌基因芯片（部件号PMDACBA1），对比野生型与同基因背景的核糖核酸酶T2家族蛋白突变菌株的基因表达特征。将鲍曼不动杆菌菌株98-37-09（野生型）或同基因背景的ACJ7（在A1S_3026位点携带EZ-Tn5插入突变）的菌体，在Luria-Bertani（LB）培养基中培养至指数生长中期，提取细菌总RNA后进行基因芯片杂交与分析。本研究旨在阐明A1S_3026的调控作用。