Fractionation of different lignocelullose by Streptomyces coelicolor A3(2) and production of secondary metabolites

The goal was to study the dfactionation of different lignocelullose (glucose, wheat bran, wheat straw) by Streptomyces coelicolor A3(2) and the corresponding production of secondary metabolites. This was performed by multi-omic experiment such as transcriptomic/metabolomic and leads to the production of new metabolites. For that, the strain Streptomyces coelicolor A3(2) was subjected to two carbon sources in triplicate (wheat bran and glucose as control). Enzymatic activities were studied at different times and the expression of CAZYmes was studied by transcriptomic in order to detect which enzymes are needed for each carbon source

本研究旨在探究天蓝色链霉菌A3(2)（Streptomyces coelicolor A3(2)）对不同木质纤维素（葡萄糖、麦麸、麦秆）的降解效果，以及该菌株在此过程中对应的次级代谢产物合成情况。本研究通过转录组学、代谢组学等多组学实验开展，最终获得了新型代谢产物。实验中将天蓝色链霉菌A3(2)接种于两种碳源培养基中，每组设置三次生物学重复，其中以葡萄糖作为对照组，麦麸为实验组。研究人员在不同培养时间节点检测了相关酶活性，并通过转录组学分析碳水化合物活性酶（CAZYmes）的表达情况，以明确不同碳源所需的特异性酶类。