CIL:34929

Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell interior at cell edge (CIL 34926) of cytochalasin D-treated human 356 fibroblasts stained with ABP-280 primary antibody and 10-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal ABP-280 at filament crossovers in the cell interior. A fluorescence image of fibroblast lamellipodia double stained with TRITC-phalloidin (red) and ABP-280 (green) can be found at (CIL 34923). Image corresponds to Figure 5h from J Cell Biol. 1999 May 31;145(5):1009-26.

成纤维细胞骨架中交联蛋白的定位。对经细胞松弛素D处理的人类356成纤维细胞，使用ABP-280一抗与10纳米金标记二抗进行染色后，于其细胞边缘处的细胞内部区域进行免疫电子显微镜（Immuno-EM）成像（对应细胞图像库（Cell Image Library，CIL）34926）。黄色金颗粒可显示出细胞内部区域肌丝交联位点处的ABP-280蛋白。可于（CIL 34923）处获取经四甲基罗丹明-鬼笔环肽（TRITC-phalloidin，红色）与ABP-280（绿色）双重染色的成纤维细胞片状伪足荧光图像。本图像对应《细胞生物学杂志（Journal of Cell Biology）》1999年5月31日刊，第145卷第5期，第1009-1026页的图5h。