Identification of Stretch Responsive microRNAs in Embyonic Mouse Cardiomyocytes. Identification of Stretch Responsive microRNAs in Embyonic Mouse Cardiomyocytes

The goal of this study was to identify microRNAs that are modulated by cyclic stretch. miRNA-Seq was performed comparing samples from cultured E16.5 mouse cardiomyocytes exposed to either cyclic stretch of 16% at 1Hz for 24h or static conditions. Overall design: 3 samples from cardiomyocytes cultured under static conditions were used as the controls. The 3 experimental samples were from cardiomyocytes exposed to cyclic stretch of 16% at 1Hz for 24h.

本研究旨在鉴定受周期性牵张调控的微小RNA（microRNAs）。采用miRNA测序（miRNA-Seq）对两组样本开展比较分析：一组为经16%幅度、1Hz频率周期性牵张处理24小时的培养E16.5小鼠心肌细胞样本，另一组为静态培养条件下的心肌细胞样本。实验整体设计：以3份静态培养的心肌细胞样本作为对照，3份实验样本则取自经16%幅度、1Hz频率周期性牵张24小时的心肌细胞。