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Diadinoxanthin (Ddx) concentration and de-epoxidation state (DES).

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https://figshare.com/articles/dataset/_Diadinoxanthin_Ddx_concentration_and_de_epoxidation_state_DES_/804437
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Aureo1a-15 and aureo1a-50P. tricornutum cultures were grown under illumination with blue (BL) and red light (RL) under limiting light (LL, QPhar = 10 µmol absorbed photons m−2 s−1) and medium light (ML, QPhar = 10 µmol absorbed photons m−2 s−1) conditions; corresponding WT data of Schellenberger Costa et al. [28] is included as reference. Ddx-concentration is given in mmol mol Chl a−1. DES was measured after 10 min of illumination with 1000 µmol photons m−2 s−1 as the ratio of diatoxanthin (Dtx) to Ddx+Dtx. Mean values are shown with standard deviation (n = 5 to 9). Mean values of aureo1a cultures marked with asterisks (*) are significantly different to the WT culture of the same culturing condition according to one-way ANOVA followed by Holm-Sidak pair wise comparison against WT as control group (p<0.05). O: no significant difference between WT and aureochrome 1a silenced strain; −: significant decrease in aureochrome 1a silenced strain compared to WT; +: significant increase in aureochrome 1a silenced strain compared to WT.

本数据集涵盖Aureo1a-15与aureo1a-50P两株隐花色素1a(aureochrome 1a)沉默的三角褐指藻(Phaeodactylum tricornutum)培养物的实验数据。上述藻株分别在限制光(Limiting Light, LL,吸收光子量子通量密度QPhar=10 µmol·m−2·s−1)与中等光(Medium Light, ML,QPhar=10 µmol·m−2·s−1)条件下,辅以蓝光(Blue Light, BL)与红光(Red Light, RL)进行培养。本研究同时纳入Schellenberger Costa等人[28]发表的野生型(Wild Type, WT)数据作为参照。 硅藻黄质(Diadinoxanthin, Ddx)的浓度单位为mmol·mol(Chl a)−1。脱环氧化状态(De-epoxidation state, DES)的测定方式为:将藻株置于光照强度1000 µmol photons·m−2·s−1的环境中照射10分钟后,以硅甲藻黄素(Diatoxanthin, Dtx)与Ddx+Dtx的摩尔比值作为表征参数。 所有实验结果均以均值±标准差的形式展示,样本量n为5至9。标注有星号(*)的aureo1a沉默菌株的均值,与相同培养条件下的野生型菌株相比存在显著性差异,该差异通过单因素方差分析(one-way ANOVA)结合以野生型为对照组的Holm-Sidak两两比较检验验证(p<0.05)。其中: O:野生型与隐花色素1a沉默菌株间无显著差异; −:隐花色素1a沉默菌株的对应指标较野生型显著降低; +:隐花色素1a沉默菌株的对应指标较野生型显著升高。
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2013-09-20
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