Do biological control agents adapt to local pest genotypes? A multi-year test across geographic scales

Parasite local adaptation has been a major focus of (co)evolutionary research on host-parasite interactions. Studies of wild host-parasite systems frequently find that parasites paired with local, sympatric host genotypes perform better than parasites paired with allopatric host genotypes. In contrast, there are few such tests in biological control systems to establish whether biological control parasites commonly perform better on sympatric pest genotypes. This knowledge gap prevents the optimal design of biological control programs: strong local adaptation could argue for the use of sympatric parasites to achieve consistent pest control. To address this gap, we tested for local adaptation of the biological control bacterium Pasteuria penetrans to the root-knot nematode Meloidogyne arenaria, a global threat to a wide range of crops. We measured the probability and intensity of P. penetrans infection on sympatric and allopatric M. arenaria over the course of four years. Our design accou..., To test for local adaptation of P. penetrans, we collected paired samples of M. arenaria and P. penetrans from six sites each year. Because the strength of local adaptation can vary with spatial scale, we collected samples from six plots within a single field in 2019 and 2020 and from six fields in 2021 and 2022. Each year, we compared the performance of P. penetrans when paired with sympatric and allopatric M. arenaria by measuring proxies for infection probability (attachment rate) and intensity (attachment load). In 2019 and 2020, we isolated the signature of parasite adaptation by comparing local adaptation of early-, mid-, and late-season parasites., , # Data from: Do biological control agents adapt to local pest genotypes? A multi-year test across geographic scales

**Includes 4 data sets, one for each year/experiment. Data sets provide the number of spores attached to individual nematodes in replicate flasks (rows) of sympatric and allopatric treatments, designated by attributes in initial columns:**

2019_LA_RAW_AR.csv data from the 2019 LA and time shift experiment, small spatial scale

2020_LA_RAW_AR.csv data from the 2020 LA and time shift experiment, small spatial scale

2021_LA_RAW_AR.csv data from the 2021 LA experiment, large spatial scale

2022_LA_RAW_AR.csv data from the 2022 LA experiment, large spatial scale

## Description of the data and file structure

**Metadata for csv data files**

| **Column Name** | **Descriptor** ...

寄生虫的局部适应一直是宿主-寄生虫互作（共）进化研究的核心焦点。野生宿主-寄生虫系统的研究常发现，与本地同域宿主基因型配对的寄生虫，其表现优于与异域宿主基因型配对的寄生虫。相比之下，生物防治系统中此类验证研究较少——即生物防治用寄生虫是否通常在同域害虫基因型上表现更优。这一知识缺口阻碍了生物防治方案的优化设计：若存在强烈的局部适应，则应选用同域寄生虫以实现稳定的害虫防控。为填补此缺口，我们测试了生物防治细菌Pasteuria penetrans对根结线虫Meloidogyne arenaria（一种危害多种作物的全球性威胁）的局部适应。我们在四年间测量了P. penetrans对同域及异域M. arenaria的感染概率与强度。我们的设计考虑了...，为验证P. penetrans的局部适应，我们每年从六个位点采集M. arenaria与P. penetrans的配对样本。由于局部适应的强度会随空间尺度变化，我们在2019和2020年从单一田块内的六个小区采集样本，2021和2022年则从六个田块采集样本。每年，我们通过测量感染概率（附着率）和强度（附着量）的替代指标，比较P. penetrans与同域及异域M. arenaria配对时的表现。2019和2020年，我们通过比较早、中、晚季寄生虫的局部适应，分离出寄生虫适应的特征。

# 数据来源：生物防治剂是否适应本地害虫基因型？跨地理尺度的多年验证

**包含4个数据集，每个对应一年/一项实验。数据集提供重复培养瓶（行）中附着于单个线虫的孢子数量，分同域和异域处理组，由初始列中的属性指定：**

2019_LA_RAW_AR.csv 2019年局部适应及时间转移实验数据，小空间尺度

2020_LA_RAW_AR.csv 2020年局部适应及时间转移实验数据，小空间尺度

2021_LA_RAW_AR.csv 2021年局部适应实验数据，大空间尺度

2022_LA_RAW_AR.csv 2022年局部适应实验数据，大空间尺度

## 数据及文件结构描述

**csv数据文件元数据**

| 列名 | 描述...