Time-resolved RNA atlas of early human T cell activation (miRNA)

MicroRNAs are central regulators of the T cell function. We explored RNA expression profiles over the initial 24 h of human CD4+ T cell activation. We found high similarity in time-resolved miRNA expression courses comparing independent activations and different donors. The detected miRNA expression patterns could be grouped into six classes only, each with a defined time course. MiR-155-5p known for its role in T cell immunity showed the most prevalent expression changes, quantified with an hourly increase of about 60 molecules/cell. As demonstrated for miRNA-155-5p, the analysis of time-resolved miRNA and mRNA expression data allowed to increase the validation rate of predicted miRNA targets to close to 90 %. Combining our time-resolved expression analysis with an absolute quantification of miRNA expression changes, gives new insights into miRNA regulatory networks and indicates the functional dominance of specific miRNAs within the early T cell activation. Early human T cell activation

微小RNA（MicroRNAs）是T细胞功能的核心调控因子。本研究针对人类CD4+ T细胞活化初始24小时内的RNA表达谱开展了分析。研究发现，在独立活化实验与不同供体样本之间，时间分辨率下的miRNA表达时序具有高度相似性。本次检测到的miRNA表达模式仅可划分为6类，每一类均具有明确的时序变化特征。因在T细胞免疫中发挥关键功能而广受关注的MiR-155-5p，其表达变化幅度最为显著，经定量分析，其表达量每小时约增加60个分子/细胞。正如miR-155-5p的分析结果所示，对时间分辨率下的miRNA与信使RNA（mRNA）表达数据进行分析，可将预测miRNA靶标的验证率提升至接近90%。将本研究的时间分辨率表达分析与miRNA表达变化的绝对定量相结合，可为miRNA调控网络提供全新认知，并揭示特定miRNA在早期T细胞活化过程中的功能主导地位。人类早期T细胞活化