Genome-wide transcriptomic analysis of Arabdiopsis mutants controling nuclear size in comparison to the wildtype

We report genome-wide transcriptomic profile of 2 week-old Arabidopsis seedlings from pwo1 and crwn1 crwn2 mutants in comparison to the wildtype (Col-0) Arabidopsis thaliana seedlings of Col-0, pwo1 and crwn1 crwn2 were grown vertically on MS plates in long-day conditions for 2 weeks. Afterwards, RNA from 3 biological replicates per genotype was isolated using RNeasy Plant Mini Kit (Qiagen) and NGS library created using TruSeq RNA Library Prep Kit v2 (Illumina). Paired-end sequencing was performed on HiSeq 2500 platform (Illumina).

本研究报道了2周龄拟南芥（Arabidopsis thaliana）幼苗的全基因组转录组谱，对pwo1、crwn1 crwn2突变体与野生型（Col-0）拟南芥幼苗进行了对比分析。将Col-0、pwo1及crwn1 crwn2基因型的材料垂直培养于MS培养基平板上，于长日照条件下培养2周。随后，针对每个基因型的3个生物学重复样本，使用Qiagen的RNeasy Plant Mini Kit提取总RNA，并采用Illumina的TruSeq RNA Library Prep Kit v2构建下一代测序（NGS）文库。最后在Illumina HiSeq 2500测序平台上完成双端测序。