Sense Cryptic transcription in histone H3 K36A, K56R and K36AK56R mutant yeast strain compared to wild-type. Saccharomyces cerevisiae

Gene expression microarray experiments to determine the existence of intragenic (cryptic) transcripts in K36A, K56R and K36AK56R mutant yeast strain compared to wildtype. Overall design: Two color experiment. Mutant/WT. Biological replicates=3. Hybridisation of same samples to strand-specific gene expression arrays.

本研究通过基因表达微阵列实验，检测K36A、K56R及K36AK56R突变酵母菌株相较于野生型菌株中基因内（隐蔽）转录本的存在情况。实验设计概述：采用双色杂交实验方案，以突变体与野生型作为对照分组，设置3次生物学重复，将相同样品用于链特异性基因表达芯片杂交。