Data from: Microsatellite primers for the Pacific Northwest endemic conifer Chamaecyparis lawsoniana (Cupressaceae)

Microsatellite primers were developed for the Pacific Northwest conifer, Chamaecyparis lawsoniana (A. Murray) Parl. (Cupressaceae), to enhance efficiencies in disease-resistance breeding and germplasm screening for gene conservation of this rare species. Using multiplexed massively parallel Illumina sequencing, we identified over 300,000 microsatellite-containing sequences from 2 million paired-end microreads. After stringent filtering and primer evaluation, we selected 11 primer pairs and used these to screen variation in 4 populations of C. lawsoniana. Loci show between three and ten repeats per locus, with an average of eight. Screening of these markers in the North American relative Callitropsis nootkatensis demonstrated limited marker transferability, but these markers could have utility in Asian species of Chamaecyparis. These genetic markers show high polymorphism and should provide a high level of individual discrimination for paternity analysis in defined pedigrees, and routine screening of wild variation in Chamaecyparis lawsoniana.

本研究针对太平洋西北针叶树种美国扁柏（Chamaecyparis lawsoniana (A. Murray) Parl.，柏科（Cupressaceae））开发微卫星引物（Microsatellite primers），以提升该珍稀物种抗病育种及基因保存相关种质筛选的效率。本研究采用多重大规模并行Illumina测序技术，从200万条双端微读段中鉴定出超过300,000条含有微卫星的序列。经严格过滤与引物评估后，最终选取11对引物，并利用其对4个美国扁柏种群的遗传变异进行筛查。每个位点的重复序列数介于3至10之间，平均为8个。将这些标记应用于北美近缘种阿拉斯加柏木（Callitropsis nootkatensis）时，仅表现出有限的标记可转移性，但这类标记有望在扁柏属（Chamaecyparis）的亚洲物种中发挥应用价值。本研究开发的这类遗传标记具有较高的多态性，可在特定谱系的父本分析以及美国扁柏野生群体遗传变异的常规筛查中实现高效的个体识别。