RNA-seq and DAP-seq analysis the function of TaMYB50

To further identify TaSWEET14d as the direct transcriptional targets of TaMYB50, DNA affinity purification sequencing (DAP-seq) was conducted to unravel its genome-wide binding sites. To elucidate the molecular mechanism by which TaMYB50 endowed increased wheat resistance to stripe rust, we conducted RNA-seq analysis of TaMYB50-OE1 leaves infected with Pst.

为进一步明确TaSWEET14d为TaMYB50的直接转录靶标，本研究开展了DNA亲和纯化测序（DNA affinity purification sequencing, DAP-seq）以解析其全基因组结合位点。为阐明TaMYB50提升小麦抗条锈病能力的分子机制，本研究对接种Pst的TaMYB50-OE1叶片进行了RNA-seq分析。