Expression profiling of mouse ILCreg cells

Investigation of global gene expression levels of ILCreg cells Gene expression profiling using sorted ILCreg cells from IL-10-GFP knockin mice in C57BL/6 background. Purity of cells was over 95% that was determined by post sorting analysis with flow cytometry. Total RNA was extracted from ILCregs using standard RNA extraction protocol, followed by DNase incubation to remove nuclear DNA. RNA quality was monitored by NanoDrop ND-1000 and RNA integrity was tested by agarose gel electrophoresis. Total RNA was amplified with Whole Transcript (WT) Pico Reagent Kit (Affymetrix). The sample preparation and microarray hybridization were performed based on the Affymetrix’s standard protocols.

调节性固有淋巴细胞（ILCreg）的全基因表达水平分析：本研究采用来自C57BL/6背景的IL-10-GFP敲入小鼠（IL-10-GFP knockin mice）的分选后调节性固有淋巴细胞开展基因表达谱分析。经流式细胞术分选后验证，细胞纯度达95%以上。采用标准RNA提取流程从调节性固有淋巴细胞中提取总RNA，随后通过脱氧核糖核酸酶（DNase）孵育去除核DNA。通过NanoDrop ND-1000检测仪监测RNA质量，并通过琼脂糖凝胶电泳检测RNA完整性。使用全转录组（Whole Transcript, WT）Pico试剂试剂盒（Affymetrix）完成总RNA扩增。样本制备及微阵列杂交实验均严格遵循Affymetrix标准实验流程进行。