Effect of overexpressing CSTA on gene expression of different nasopharyngeal carcinoma (NPC) cells. Effect of overexpressing CSTA on gene expression of different nasopharyngeal carcinoma (NPC) cells

To investigate the function of CSTA, we established CSTA-overexpressing cell lines.We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different NPC cells . Overall design: To elucidate the molecular mechanisms of CSTA in suppressing NPC cellular motility, we performed high-throughput sequencing of S18 and 5-8F cells with CSTA overexpression or vector control.

为探究半胱氨酸蛋白酶抑制剂A（CSTA）的生物学功能，本研究构建了过表达CSTA的细胞系。随后，我们采用4种不同鼻咽癌（Nasopharyngeal Carcinoma, NPC）细胞的RNA测序（RNA-seq）数据开展基因表达谱分析。
实验设计概述：为阐明CSTA抑制鼻咽癌细胞迁移能力的分子机制，我们对过表达CSTA及空载对照的S18与5-8F细胞进行了高通量测序。