Single cell RNA-seq analysis of endothelial cells during diabetic atherosclerosis. Single cell RNA-seq analysis of endothelial cells during diabetic atherosclerosis

Single-cell RNA sequencing (scRNA-seq) was performed on the CD31-mircrobeads enriched endothelial cells isolated from aorta and heart from eight-week-old, male Ldlr-/- mice fed standard chow (Chow) or diabetogenic high-fat diet with 0.15% cholesterol (DDC) diet for 12 weeks. This study report the changes of EC subpopulations, fractions, transcriptomic and metabolic profiles during diabetic atherosclerosis. Overall design: Examination of the transcriptomic profiles of endothelial cells isolated from aorta and heart pooled from three Ldlr-/- mice fedstandard chow (Chow) or diabetogenic high-fat diet with 0.15% cholesterol (DDC) diet for 12 weeks

本研究对8周龄雄性Ldlr⁻/⁻小鼠的主动脉与心脏来源、经CD31-mircrobeads富集的内皮细胞开展单细胞RNA测序（Single-cell RNA sequencing，scRNA-seq），实验小鼠分为两组，分别饲喂标准饲料（Chow）或含0.15%胆固醇的致糖尿病高脂饮食（DDC diet），干预时长为12周。本研究报道了糖尿病性动脉粥样硬化进程中内皮细胞亚群、细胞占比、转录组及代谢谱的变化。整体实验设计：对三只分别饲喂标准饲料（Chow）或含0.15%胆固醇的致糖尿病高脂饮食（DDC diet）12周的Ldlr⁻/⁻小鼠，取其主动脉与心脏组织中分离得到并混合的内皮细胞，对其转录组谱进行检测。