The species richness of the Salix viminalis rhizosphere. 16S rRNA profiling of rhizosphere

Manipulating the rhizosphere microbiome to enhance plant stress tolerance is an environmentally friendly technology and a renewable resource to restore degraded environments. We considered the Salicornia europaea rhizosphere and the ability of the phytoremediation plant Salix viminalis to recruit its beneficial microbiome to mediate the pollution stress at the Stebnyk mine tailings storage. The tailings contain large amounts of brine salts and heavy metals that contaminate the groundwater and surrounding areas, changing soil biogeochemistry and causing increased erosion. We performed the 16S rRNA profiling of the V3-V4 region by using Pro341F and Pro805R primer pair, to assess the species richness of the endophytic bacterial community of Salix viminalis roots grown in soils originating from the Stebnyk mine tailings storage. Thirty-six (36) individual cuttings were examined at a gradient of the Stebnyk mine tailing representing three contamination levels. Salix viminalis was grown either in unsupplemented soil samples or in soil samples enriched with slurries from the S. europaea rhizosphere. Our results show that biodiversity was decreased across the contamination zones and that Salicornica europaea supplementation significantly increased the species richness.

调控根际微生物组（rhizosphere microbiome）以提升植物抗逆性，是一类环境友好型技术，同时也是修复退化环境的可再生资源。本研究以盐角草（Salicornia europaea）根际菌群为研究对象，并利用修复型植物蒿柳（Salix viminalis）招募有益微生物组的能力，以缓解斯特布尼克尾矿库（Stebnyk mine tailings storage）的污染胁迫。该尾矿库含有大量卤盐与重金属，可污染地下水及周边区域，改变土壤生物地球化学特征，并加剧土壤侵蚀。本研究采用Pro341F与Pro805R引物对，对16S rRNA基因V3-V4可变区进行谱分析，以此评估取自斯特布尼克尾矿库的土壤中生长的蒿柳根系内生细菌群落的物种丰富度。本实验共检测36个独立插条样本，样本梯度覆盖斯特布尼克尾矿的三种污染水平。蒿柳分别种植于未添加外源基质的土壤样本，以及添加了盐角草根际菌群浆液的土壤样本中。研究结果显示，各污染区域的生物多样性均出现下降，而盐角草添加处理可显著提升群落的物种丰富度。