Gene expression data from Mt/Gigi vs Mt root tissues

Most vascular flowering plants have the ability to form mutualistic associations with soil fungi from the Glomeromycota. The resulting symbiosis is called an arbuscular mycorrhiza and they are widespread in terrestrial ecosystems throughout the world. Significant alteration occurs at physiological and molecular levels in both symbionts. To gain a better understanding of the AM symbiosis, we use a 16000 feature oligonucleotide based array to examine gene expression in an arbuscular mycorrhizal symbioses, M. truncatula/Gigaspora gigantea. Keywords: Medicago truncatula, Mycorrhizal, Gigaspora gigantea, microarray profiling 28 days after inoculation, the Mt/Gigi and mock-inoculated (Mt) root tissues were harvested for RNA extraction and hybridized to the Medicago Genome Oligo Set Version 1.0 microarray. Six slides including three biological replicates and three dye-swap labeling replicates were hybridized for the experiment.

绝大多数维管束开花植物均可与球囊霉门（Glomeromycota）的土壤真菌形成互利共生关系。由此形成的共生体系被称为丛枝菌根（arbuscular mycorrhiza），该类共生关系在全球陆地生态系统中广泛分布。共生双方的生理与分子层面均会发生显著改变。为深入解析丛枝菌根（AM）共生机制，本研究采用包含16000个特征探针的寡核苷酸微阵列（oligonucleotide based array），对丛枝菌根共生体系蒺藜苜蓿（Medicago truncatula）/巨孢囊霉（Gigaspora gigantea）的基因表达情况进行检测。关键词：蒺藜苜蓿（Medicago truncatula）、菌根、巨孢囊霉（Gigaspora gigantea）、接种后28天的微阵列基因表达谱分析；实验分别收集Mt/Gigi共生组与模拟接种（mock-inoculated）对照组的Mt根组织，提取总RNA后与蒺藜苜蓿基因组寡核苷酸芯片版本1.0（Medicago Genome Oligo Set Version 1.0）进行杂交。本实验共设置6张杂交芯片，包含3次生物学重复与3次染料互换标记重复。