ADK interacts with SnRK1 and SnRK1-KD in yeast cells.

The indicated bait proteins were expressed as GAL4 DNA binding domain fusions, and the prey proteins as GAL4 activation domain fusions, in yeast Y190 cells. SnRK1 indicates the full-length protein and SnRK1-KD denotes the kinase domain. Chloramphenicol acetyl transferase (CAT) and p53 were negative controls. Interaction was indicated by cell growth on medium lacking histidine and containing 50 mM aminotriazole. As an additional indicator of interaction, colonies were monitored for LacZ activity (blue color) using a filter-lift assay.

将指定的诱饵蛋白构建为GAL4 DNA结合结构域（GAL4 DNA binding domain）融合蛋白，猎物蛋白则构建为GAL4激活结构域（GAL4 activation domain）融合蛋白，共同转化至酵母Y190细胞中。其中SnRK1代表全长蛋白，SnRK1-KD指代其激酶结构域。氯霉素乙酰转移酶（CAT）与p53作为阴性对照。蛋白互作可通过在缺失组氨酸且添加50mM氨基三唑的培养基上的细胞生长情况进行判定。作为互作的另一检测指标，通过滤膜裂解实验（filter-lift assay）对菌落的LacZ活性（蓝色显色结果）进行监测。