USP4 depletion-driven RAB7A ubiquitylation impairs autophagosome-lysosome fusion and aggravates periodontitis

In exploring the pathogenesis of periodontitis, we collected clinical gingival tissues from healthy donors and patients with severe periodontitis for RNA-Seq analysis, and calculated the significant statistical correlation values (R) between autophagy-related genes and inflammation-related genes in human gingival tissues, which could inform a potential therapeutic target for the treatment of periodontitis. To explore the probable relationship between autophagy-related genes and inflammation-related genes, Clinical human gingiva tissues were collected from severe periodontitis patients (Stage IIIC) and healthy individuals. We then performed gene expression profiling analysis using data obtained from RNA-Seq of these samples.

本研究为探究牙周炎的发病机制，收集了健康供体与重度牙周炎患者的临床牙龈组织以开展RNA测序（RNA-Seq）分析，并计算了人牙龈组织中自噬相关基因（autophagy-related genes）与炎症相关基因（inflammation-related genes）之间的显著统计相关值（R），该结果可为牙周炎的治疗提供潜在治疗靶点。为探究自噬相关基因与炎症相关基因间的潜在关联，我们从重度牙周炎（IIIC期）患者及健康个体中采集临床牙龈组织，随后基于上述样本的RNA测序数据开展基因表达谱分析。