Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing. Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing

We performed single-cell RNA sequencing on bone marrow derived, cultured dendritic cells after CRISPR-knockout of Ndrg2, after vitamin D3 treatment or no treatment. In addition we performed single-cell RNA sequencing of wounds that had been treated with hydrogels seeded with Ndrg2-KO dendritic cells, control (untreated) dendritic cells or blank hydrogels. Overall design: scRNA-seq was performed on murine cells collected from parabiome excisional wounds. Cells labeled as GFP+ were isolated and sequenced to analyze cells in the circulation that hone to the wound upon treatment.

我们对经CRISPR敲除Ndrg2、经维生素D3（vitamin D3）处理或未处理的骨髓来源培养树突状细胞（dendritic cells），进行了单细胞RNA测序（single-cell RNA sequencing，scRNA-seq）。此外，我们还对分别接种了Ndrg2敲除型树突状细胞、对照（未处理）树突状细胞或空白水凝胶（hydrogels）的伤口进行了单细胞RNA测序。总体实验设计：对从联体切除伤口中收集的小鼠细胞进行单细胞RNA测序。我们分离并测序了标记为GFP+的细胞，以分析经处理后向伤口归巢的循环系统细胞。