RNA sequencing of control and dystrophin-mutant engineered heart tissues from human induced pluripotent stem cell-derived cardiomyocytes

We report the transcriptomic differences between engineered heart tissues generated from hiPSCs with a dystrophin-truncating mutation and an isogenic control. We find that the dystrophin mutation induces profound differences at the transcriptomic level and illuminates potential disease-causing mechanisms. Overall design: RNA sequencing of control and dystrophic engineered heart tissues

本研究报道了由携带肌营养不良蛋白截短突变的人类诱导多能干细胞（human induced pluripotent stem cells, hiPSCs）构建的工程化心脏组织，与同基因对照样本之间的转录组差异。研究发现，肌营养不良蛋白突变可在转录组水平引发显著差异，并揭示了潜在的致病机制。整体实验设计：对对照及肌营养不良型工程化心脏组织进行RNA测序。