Expression data from mouse embryonic neural stem cells

Neural stem cells from different brain regions show differencies in gene expression patterns and physiological functions. We used microarrays to find differential gene expression between the neuralstem cells from the subventricular zone of lateral ventricle and the subventricular zone of third ventricle. Cultured neural stem cells from embryonic day 17.5 mouse embryos were selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain embryonic neural stem cell cultures from three indipendent females.

不同脑区的神经干细胞(Neural stem cells)在基因表达模式与生理功能上存在差异。本研究采用微阵列(microarrays)技术，筛选侧脑室室管膜下区与第三脑室室管膜下区来源的神经干细胞之间的差异表达基因。我们选取胚胎17.5天小鼠胚胎来源的体外培养神经干细胞进行RNA提取，并在Affymetrix微阵列上开展杂交反应。本研究旨在获取3只独立雌性小鼠来源的胚胎神经干细胞培养物。