Pre-analytical changes of mRNA Gene Expression as the result from prolonged storage
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE1347
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Impact of pre-analytical handling on Bone Marrow (BM) mRNA Gene Expression: We have investigated the impact of RNA extraction protocols and time delays (24 and 48h) between sample aspiration and RNA extraction on RNA quality and gene expression profiles. Intact RNA can be extracted from BM samples stored at room temperature for up to 48h after aspiration. However, storage of BM has dramatic effects on mRNA expression of individual transcripts. Keywords: time-course
分析前处理对骨髓(Bone Marrow, BM)mRNA基因表达的影响:本研究探讨了RNA提取方案,以及样本抽吸至RNA提取之间的24小时、48小时时间延迟对RNA质量与基因表达谱的影响。在样本抽吸后,可从室温储存最长达48小时的骨髓样本中提取到完整RNA。但骨髓样本储存对单个转录本的mRNA表达具有显著影响。关键词:时间进程(time-course)
创建时间:
2018-12-13



