Expression profiles of beta-hematin (BH)- or 4-hydroxy-2-nonenal (HNE)-treated RAW 264.7 cells. Mus musculus

Hemozoin phagocytosis results in immunomodulation. In the present study, gene expression profiles were analyzed to identify transcriptional changes associated with two of the individual components of Hz in a model macrophage cell line. LPS-stimulated RAW 264.7 cells were exposed to the synthetic form of Hz, β-hematin (BH, 0.1 mg/mL) and HNE (35 μM) for 6 or 24 h. Keywords: variable treatment Overall design: Three biological replicates were analyzed for each 3 treatment types (HNE, BH, bead) in LPS stimulated cells in addition to 3 replicates of untreated LPS stimulated cells (control). Each replicate was composed of 6 pooled wells from a 6-well plate. In order to identify transcriptional changes dependent on interactions of BH rather than those due to phagocytosis, differentially expressed mRNAs were controlled by a corresponding particulate latex bead challenge.

疟色素（Hemozoin）的吞噬作用可引发免疫调控。本研究通过分析基因表达谱，旨在鉴定模型巨噬细胞系中与疟色素（Hz）两种单独组分相关的转录组变化。经脂多糖（LPS）刺激的RAW 264.7细胞分别用合成型疟色素（即β-血红素，BH，0.1 mg/mL）以及HNE（35 μM）处理6小时或24小时。
关键词：可变处理
实验设计：针对经LPS刺激的细胞，我们对HNE、BH、乳胶微珠（latex bead）这3种处理类型各设置3次生物学重复；此外还设置了3次仅经LPS刺激而未接受其他处理的细胞作为对照。每个生物学重复由6孔板中6个混合收集的孔的细胞样本组成。为了筛选由BH介导的相互作用所引发的转录组变化（而非由吞噬作用本身导致的变化），我们通过设置相应的乳胶微珠刺激对照来校正差异表达的mRNA。