Young and Old Mouse Parabiosis Hematopoietic Stem Cell RNA Sequencing. Young and Old Mouse Parabiosis Hematopoietic Stem Cell RNA Sequencing

RNA sequencing of hematopoietic stem cells isolated from young (8-12 week) and old (24-27 month) BL6 mice from experimental groups that are intended to describe the transcriptomic consequences of parabiosis on the function of old hematopoietic stem cells. Overall design: 35 samples were processed, of which 30 met library and sequencing quality thresholds to be included in our analysis. These 30 samples represent eleven different biological groups. Some groups have only two biological replicates (YISOB6, YISOBJ, OHETB6X), but most have a minimum of three biological replicates per group (YB6CNT, YBJCNT, YHETBJ, OHETB6, OISOB6, OB6CNT). Two biological groups only had one replicate (OISOBJ and OBJCNT) and were not utilized for statistical differential expression testing unless they were merged with another sample group.

本数据集针对从年轻（8~12周龄）与老年（24~27月龄）BL6小鼠中分离的造血干细胞（hematopoietic stem cell）开展RNA测序，实验分组旨在阐明联体共生（parabiosis）对老年造血干细胞功能的转录组学影响。整体实验设计：共计处理35份样本，其中30份符合建库与测序质量阈值，纳入后续分析。该30份样本对应11个不同的生物学重复组。部分组别仅含2个生物学重复（YISOB6、YISOBJ、OHETB6X），但绝大多数组别至少包含3个生物学重复（YB6CNT、YBJCNT、YHETBJ、OHETB6、OISOB6、OB6CNT）。另有2个生物学组仅各含1个生物学重复（OISOBJ与OBJCNT），除非与其他样本组合并，否则无法用于统计学差异表达分析。