Elucidation of the mechanism behind the potentiating activity of baicalin against Burkholderia cepacia complex biofilms

Burkholderia cenocepacia J2315 biofilms were described to have an increased susceptibility towards tobramycin when baicalin hydrate was added to the treatment. The goal of this transcriptomic analysis is to elucidate the effect of baicalin hydrate on gene expression levels when added to tobramycin treatment. Therefore, biofilms were grown for 24 hours and treated for another 24 hours with either tobramycin (TOB) (1024ug/ml), baicalin hydrate (BH) (250uM) or a combination of both. Also, an untreated control (physiological saline) was included. This experiment was repeated twice, so three biological replicates per treatment were included for RNAsequencing.

已有研究表明，当向处理体系中添加黄芩苷水合物（baicalin hydrate）时，洋葱伯克霍尔德菌（Burkholderia cenocepacia）J2315生物被膜（biofilms）对妥布霉素（tobramycin）的敏感性显著升高。本转录组分析（transcriptomic analysis）旨在阐明黄芩苷水合物联合妥布霉素处理时，对菌体基因表达水平（gene expression levels）的调控效应。实验中，生物被膜先培养24小时，随后分别以妥布霉素（TOB，1024μg/ml）、黄芩苷水合物（BH，250μM）或二者联合制剂处理24小时，同时设置生理盐水处理的空白对照组。本实验重复开展两次，因此每种处理组均包含3个生物学重复样本用于RNA测序（RNAsequencing）。