A unique epigenomic landscape defines CD8+ tissue-resident memory T cells [Perturb-seq]. A unique epigenomic landscape defines CD8+ tissue-resident memory T cells [Perturb-seq]

scATAC-seq datasets of CD8+ T cells in LCMV and HSV infections Overall design: 10 libraries (5 populations x 2 cohort replicates) were created for Perturb-seq, pairing gene expression and sgRNA counts per cell in a massively-parallel assay.

本数据集为淋巴细胞性脉络丛脑膜炎病毒（Lymphocytic Choriomeningitis Virus, LCMV）与单纯疱疹病毒（Herpes Simplex Virus, HSV）感染状态下CD8阳性T细胞的单细胞转座酶可及性测序（single-cell Assay for Transposase-Accessible Chromatin using sequencing, scATAC-seq）数据集。实验设计总览：共构建10个测序文库（5个细胞群×2个队列重复样本），采用扰动测序（Perturb-seq）技术，通过大规模并行检测实验实现单个细胞内基因表达与单引导RNA（single guide RNA, sgRNA）计数的配对关联分析。