Spontaneous resistance to phage NPV1 confers increased daptomycin susceptibility in Enterococcus faecalis OG1RF

Enterococcus faecalis is a Gram-positive opportunistic pathogen that inhabits the gastrointestinal tract. Because of the high frequency of antibiotic resistance among Enterococcus clinical isolates, interest in using phage to treat enterococcal infections and to decolonize high-risk patients for antibiotic-resistant Enterococcus is rising. Bacteria can evolve phage resistance, but there is little published information on these mechanisms in E. faecalis. In this report, we identified genetic determinants of resistance to phiNPV1 and their effects on E. faecalis susceptibilities to daptomycin and sodium chloride-induced osmotic stress.

粪肠球菌（Enterococcus faecalis）是一种定植于胃肠道的革兰氏阳性机会致病菌。鉴于肠球菌临床分离株的抗生素耐药性检出率居高不下，利用噬菌体治疗肠球菌感染，并对携带抗生素耐药性肠球菌的高危患者实施去定植的研究兴趣日益浓厚。细菌可进化出噬菌体抗性，但目前关于粪肠球菌的此类抗性机制的公开报道仍较为有限。本研究明确了针对噬菌体phiNPV1的抗性遗传决定因子，及其对粪肠球菌在达托霉素与氯化钠诱导渗透压应激下的敏感性的影响。