Effects of upregulated JAK/STAT signaling on gene expression in Drosophila airway epithelial cells

Chronically activated JAK/STAT signaling leads to several lung diseases, including lung cancer, asthma, and chronic obstructive pulmonary disease. We used the fruit fly Drosophila melanogaster to elucidate the molecular processes which transform elevated JAK/STAT signaling in airway epithelial cells into pathology. Overall design: We performed gene expression profiling analysis using data obtained from RNA-seq of 2 different tracheal tissues of Drosophila 3rd instar larvae at one time point.

持续激活的JAK/STAT信号通路可引发多种肺部疾病，包括肺癌、哮喘与慢性阻塞性肺疾病。
本研究以黑腹果蝇（Drosophila melanogaster）为模式生物，阐明将气道上皮细胞中JAK/STAT信号通路活性升高转化为病理状态的分子机制。
实验总体设计：我们针对果蝇三龄幼虫的2种不同气管组织开展RNA测序（RNA-seq），并基于获取的数据集进行基因表达谱分析，所有样本采集自单一时间点。