Histological, metabolomic, and transcriptomic differences in fir trees from a peri-urban forest under chronic ozone exposure

The objective of the project is to determine transcripts associated with trees (Abies divino) symptomatic and asymptomatic due to tropospheric ozone in a forest naturally exposed to ozone. Data comes from RNAseq sequencing. The 18 sequencing libraries from poly(A)+ enriched RNA were prepared, and then sequenced in a Hi-Seq 4000 in a 150PE sequencing lane at the University of Berkeley, USA

本项目的研究目标为：明确在自然暴露于对流层臭氧（tropospheric ozone）的森林中，与受臭氧胁迫出现症状及无症状的冷杉（Abies divino）相关的转录本（transcripts）。本研究数据来源于RNA测序（RNA-seq）。实验共制备了18份以聚腺苷酸富集RNA（poly(A)+ enriched RNA）为模板的测序文库，随后于美国加州大学伯克利分校（University of Berkeley）的Hi-Seq 4000测序平台的150PE测序泳道中完成测序。