Single-cell analysis of mouse pituitaries

Using the single-cell sequencing technology, the authors aim to make a globally construction of a single-cell cell atlas covering young and old pituitary cells. Moreover, the sorted Ai6+ cells from pituitary of Igha-Cre; Ai6 were also analyzed by single-cell sequencing. Overall design: The young and old mouse pituitary cells, as well as sorted Igha-Cre; Ai6+ pituitary cells were captured and analyzed using the 10× Chromium platform.

本研究采用单细胞测序技术，旨在构建一套覆盖幼年与老年垂体细胞的全局单细胞细胞图谱（single-cell cell atlas）。此外，研究还对从Igha-Cre; Ai6小鼠垂体中分选得到的Ai6+细胞开展了单细胞测序分析。实验整体设计：本研究使用10× Chromium平台，对幼年与老年小鼠垂体细胞，以及分选获得的Igha-Cre; Ai6+垂体细胞进行捕获与测序分析。