Expression data of control (F/F) and Snai1 knockout (KO) Flk1+/- cells

Snai1 is a master factor of epithelial to mesenchymal transitioin (EMT), however, its role in embryonic stem cell (ESC) differentiation and lineage commitment remains undefined. We used microarrays to compare the global programme of gene expression between control and Snai1 knockout Flk1+ and Flk1- cells sorted from 4 day EBs. Control and Snai1 knockout ESCs were cultured as embryoid bodies in spotaneous differentiation media, 4 days EBs were dissociated and sorted by anti-Flk1 antibody to separated Flk1+ and Flk1- cells, total RNA were collected for Affymetrix microarrays

Snai1是上皮间质转化（epithelial to mesenchymal transition, EMT）的核心调控因子，但其在胚胎干细胞（embryonic stem cell, ESC）分化及谱系定型中的功能仍未明确。本研究采用基因芯片技术，对比了对照组与Snai1敲除组中，从4天胚状体（embryoid bodies, EBs）分选得到的Flk1阳性（Flk1+）与Flk1阴性（Flk1-）细胞的全基因表达谱。具体实验流程为：将对照组与Snai1敲除的胚胎干细胞培养于自发分化培养基中形成胚状体，待培养至第4天时，将胚状体解离，通过抗Flk1抗体分选获得Flk1+与Flk1-细胞，随后收集总RNA用于Affymetrix基因芯片检测。